Dr. Reza Yousefi (Associate Professor of Biochemistry)

Department
Biology
About

Professional Summary

- Our research group is interested in the role of mutation and chemical modification on structural/functional variation and aggregation of lens crystallins and human insulin. Crystallin proteins are pathogenic targets for various chemical modifications and mutations which ultimately affect the proper function and transparency of eye lens (Fig.1). Stability and aggregation of insulin is currently a big medical and biotechnological challenge (Fig.2). Site-directed mutagenesis is an important technique to obtain new insulin molecules with increased stability and lower propensity for aggregation.

- We are interested also in the inhibitory potential of synthetic ligands against alpha-glucosidase and alpha-amylase enzymes which are potential targets for controlling post-prandial hyperglycemia in diabetic patients (Fig.3).

- Other research direction in our lab is to examine anticancer and DNA/protein binding properties of novel synthetic platinum complexes. The anticancer activity of Pt complexes is believed to be result of their interaction to cellular DNA and their ability to distort  DNA structure. Also, non-specific interaction of these complexes with protein can explain their potential side effects  (Fig.4)

- We are using various techniques including cloning, mutagenesis and different types of chromatography, UV-Vis spectroscopy, fluorescence measurement, circular dichroism (CD), differential scanning calorimetery (DSC), dynamic light scattering (DLS), various types of electrophoretic assessments and western blot, microscopic visualization and ELISA, insilco analysis and cell culture techniques in our studies. All of these experiments require contributions from the disciplines of  biochemistry, molecular biology, chemical biology and organic chemistry.

Research group

 

PhD students 

Kazem Khoshaman

General topic: Role of mutation in structure and function   of recombinant alphaA-crystallin.

 

Maryam Ghahramani 

General topic: Role of mutation in structure and function of recombinant alpha B-crystallin.

 

 

Mohsen Akbarian

General topic: Role of mutation in structure, stability and aggregation of recombinant insulin.

 

Musa Amirahmadi

General topic: Concurrent trainig and lens opacity: The preventive role of antioxidant compounds

 

                  Mohammad Bagher Shahsavani

 General topic: Role of mutation in structure, stability and  aggregation of recombinant insulin.

MSc students

 

Mahtab Hafizi

General topic: Role of mutation in structure and function of recombinant alpha B-crystallin.

Pezhman Ashou

General topic: Anticancer activity of Platinum complexes and their interaction with HSA and DNA.

 

 

 

 

 

 

 

 

Collaborations

A) Local collaboration

1) Professor A. Khalafi-Nezhad (Department of Chemistry, Shiraz University).

2) Professor M. Rashidi (Department of Chemistry, Shiraz University).

3) Professor S.M. Nabavizadeh (Department of Chemistry, Shiraz University).

4) Professor A.Oryan (Department of Pathology, School of Veterinary Medicine, Shiraz University).

5) Dr. A.Niazi (Institute of Biotechnology,  Shiraz University)

6) Professor M.M. Alavianmehr (Department of Chemistry, Shiraz University of Technology).

7) Professor Y.Ghasemi (Department of Pharmaceutical Biotechnology, Shiraz University of Medical Sceinces).

8) Dr. A.M Tamaddon (Department of Pharmaceutical Biotechnology, Shiraz University of Medical Sceinces).

B) National collaboration

1) Professor A.A.Moosavi-Movahedi (Institute of Biochemistry and Biophysics, Tehran University).

2) Dr A.Taheri-Kafrani (Department of Biotechnology, Isfahan University).

C) International collaboration

1) Professor B.I.Kurganov (Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia).

2) Professor Luciano Saso (Department of Physiology and Pharmacology, Sapienza University of Rome, Rome, Italy.

 

Research Interests
  •  Protein Structure, Folding and Stability   Protein Aggregation and  Role of Chaperones  Cloning, Expression  and Purification of Reccombinant Proteins  Enzyme Inhibition Assessment   Bioactive Peptides   Biological Activity of Synthetic Ligands, Organometallic Complexes and Nanomaterials.
Courses
Publications
  • 1) Moghadam SS, Oryan A, Kurganov BI, Tamaddon AM, Alavianehr MM, Moosavi-Movahedi AA, Yousefi R (2017), The structural damages of lens crystallins induced by peroxynitrite and methylglyoxal, two causative players in diabetic complications and preventive role of lens antioxidant components, International Journal of Biological Macromolecules 103, 74-88. IF=3.671

    2) Khoshaman K, Yousefi R, Niazi A, Oryan A, Moosavi-Movahedi AA, Kurganov BI (2017), Importance of the positively charged residue at position 54 to the chaperoning function, conformational stability and amyloidogenic nature of human αA-crystallin, The Journal of Biochemistry. IF=2.082

    3) Khoshaman K, Yousefi R, Moosavi-Movahedi AA (2017), Protective role of antioxidant compounds against peroxynitrite-mediated modification of R54C mutant αA-crystallin, Archives of biochemistry and biophysics 629, 43-53. IF=3.165

    4) Sadeghian T, Tavaf Z, Oryan A, Shokouhi R, Pourpak Z, Moosavi-Movahedi AA, Yousefi R (2017), Structure, chaperone-like activity and allergenicity profile of bovine caseins upon peroxynitrite modification: New evidences underlying mastitis pathomechanisms, International Journal of Biological Macromolecules. IF=3.671

    5) Yousefi R, Saba S, Ghahramani M (2017), A comparative study of the impact of calcium ion on structure, aggregation and chaperone function of human αA-crystallin and its cataract-causing R12C mutant, Protein and peptide letters. IF=0.964

    6) Najjar FM, Ghadari R, Yousefi R, Safari N, Sheikhhasani V, Sheibani N, Moosavi-Movahedi AA (2017), Studies to reveal the nature of interactions between catalase and curcumin using computational methods and optical techniques, International journal of biological macromolecules 95, 550-556. IF=3.671

    7) Khoshaman K, Masoudi R, Hemmateenejad B, Yousefi R (2017), The structural alteration and aggregation propensity of glycated lens crystallins in the presence of calcium: Importance of lens calcium homeostasis in development of diabetic cataracts, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 170, 174-183. IF= 2.536

    8) Asadi Z, Mosallaei H, Sedaghat M, Yousefi R (2017), Competitive binding affinity of two lanthanum (III) macrocycle complexes toward DNA and bovine serum albumin in water, Journal of the Iranian Chemical Society, 1-19. IF=1.407

    9) Yousefi R, Ferdowsi L, Tavaf Z, Sadeghian T, Tamaddon AM, Moghtaderi M, Pourpak Z, (2107), Evaluation of Structure, Chaperone-Like Activity and Allergenicity of Reduced Glycated Adduct of Bovine β-casein, Protein and peptide letters 24 (1), 46-55. IF=0.964

    10) Khoshaman K, Yousefi R, Tamaddon AM, Abolmaali SS, Oryan A, Moosavi-Movahedi AA, Kurganov BI, The impact of different mutations at Arg54 on structure, chaperone-like activity and oligomerization state of human αA-crystallin: The pathomechanism underlying congenital cataract-causing mutations R54L, R54P and R54C, Biochim Biophys Acta. 2017, pii: S1570-9639 (17) 30018-3. doi: 10.1016/j.bbapap.2017.02.003, IF=3.06

    11) Zafaranchi ZM S, Khoshaman K, Masoudi R, Hemmateenejad B, Yousefi R (2017) The structural alteration and aggregation propensity of glycated lens crystallins in the presence of calcium: Importance of lens calcium homeostasis in development of diabetic cataracts, Spectrochim Acta A Mol Biomol Spectrosc. 170: 174-83. IF=2.65.

    12) Shahsavai MB, Ahmadi S, Dadkhah-Aseman M, Nabavizadeh SM, Alavianmer MM, Yousefi R (2016) Comparative study on the interaction of two binuclear Pt (II) complexes with human serum albumin: Spectroscopic and docking simulation assessments, Journal of Photochemistry & Photobiology, B: Biology, In Press. IF=3.18

    13) Moafian Z, Khoshaman K, Oryan K, Kurganov BI, Yousefi R (2016) Protective effects of acetylation on the pathological reactions of the lens crystallins with homocysteine thiolactone. PLOS ONE, In Press. IF=3.23.

    14) Anbaraki A, Khoshaman K, Ghasemi Y, Yousefi R (2016) Antioxidant defense mechanism against riboflavin-mediated sunlight damaging of lens crystallins, International Journal of Biological Macromolecules, 91: 895-904. IF=3.13

    15) Mehraban MH, Odooli S, Yousefi R, Roghanian R, Motovali-Bashi M, Moosavi-Movahedi AA, Ghasemi Y (2016) The interaction of beta-lactoglobulin with ciprofloxacin and kanamycin; a spectroscopic and molecular modeling approach, Journal of Biomolecular Structure and Dynamics, 19: 1-11.    IF=2.30

    16) Javadi S, Yousefi R, Hosseinkhani S, Tamaddon A, Uversky V (2016) Protective effects of carnosine on dehydroascorbate-induced structural alteration and opacity of lens crystallins: Important implications of carnosine pleiotropic functions to combat cataractogenesis.  Journal of Biomolecular Structure and Dynamics, 19: 1-19.  IF=2.30

    17) Shahsavani MB, Ahmadi SH, Dadkhah Aseman M, Nabavizadeh SM, Rashidi M, Asadi Z, Erfani N, Ghasemi A, Saboury AA, Niazi A, Bahaoddini A, Yousefi R (2016) Anticancer activity assessment of two novel binuclear platinum (II) complexes, Journal of Photochemistry & Photobiology B, 2, 161: 345-354. IF=3.03

    18) Ghahramani M, Yousefi R, Khoshaman K, Moghadam SS, Kurganov BI (2016) Evaluation of structure, chaperone-like activity and protective ability of peroxynitrite modified human α-Crystallin subunits against copper-mediated ascorbic acid oxidation, Int J Biol Macromol, 87: 208-21. IF=3.13

    19) Rastegari B, Karbalaei-Heidari H, Yousefi R, Zeinali S, Nabavizadeh S (2016) Interaction of prodigiosin with HSA and β-Lg: Spectroscopic and molecular docking studies, Bioorganic & Medicinal Chemistry, 24 (7): 1504-12. IF=2.92

    20) Bahmari F, Panahi F, Daneshgar F, Yousefi R, Shahsavani M, Khalafinezhad A (2016) Synthesis of new α-aminophosphonate derivatives incorporating benzimidazole, theophylline and adenine nucleobases using L-cysteine functionalized magnetic nanoparticles (LCMNP) as magnetic reusable catalyst: Evaluation of their anticancer activities, RSC Advances, 6:5915-5924. IF=3.84

    21) Ragerdi kashani M, Yousefi R, Akbarian M, Alavianmehr M, Ghasemi Y (2016) Structure, chaperone activity and aggregation of wild-type and R12C mutant alphaB-crystallins in the presence of thermal stress and calcium ion; implications for the role of calcium in cataract pathogenesis, Biochemistry (Moscow), 81(2), 122-134. IF=1.42

    22) Hajari S, Masoudi R, Javadi S, Hemmateenejad B, Yousefi R (2016) The structural alteration and aggregation of bovine lens gamma-crystallin by homocysteinylation; the pathomechanism underlying cataract development during hyperhomocysteinimia, Proteins and Peptide Letters, 23(1):78-86. IF=1.06

    23) Yousefi R, Jamshidi M, Shahsavani M, Nabavizadeh S M, Golbon Haghighi M, Rashidi M, Taheri-Kafrani A, Niazi A, Keshavarz F, Alavinamehr MM, (2016) Study on the interaction of three structurally related cationic Pt (II) complexes with human serum albumin: importance of binding affinity and denaturing properties, Journal of the Iranian Chemical Society, 13(4):617-630. IF=1.30

    24) Yousefi R, Javadi S, Amirghofran S, Oryan A, Moosavi-Movahedi A (2016) Assessment of structure, stability and aggregation of soluble lens proteins and alpha-crystallin upon non-enzymatic glycation: The pathomechanisms underlying cataract development in diabetic patients, International Journal of Biological Macromolecules, 82:328-38. IF=3.13

    25) Yousefi R, Taheri B, Alavi P, Shahsavani M, Asadi Z, Ghahramany M, Niazi A, Alavian mehr M, Moosavi-Movahedi A (2016) Aspirin-mediated acetylation induces structural alteration and aggregation of bovine pancreatic insulin, Journal of Biomolecular Structure & Dynamics, 34(2): 362-375.   IF=2.30                               

    26) Yousefi R, Taheri-Kafrani A, Nabavizadeh S, Pouryasin Z, Shahsavani M, Khoshaman K, Rashidi M (2015) The binding assessment with human serum albumin of novel six-coordinate Pt(IV) complexes, containing bidentate nitrogen donor/methyl ligands, Molecular Biology Research Communications 4:167-179.

    27) Khoshaman K, Yousefi R, Saso L, Moosavi-Movahedi A (2015) The impact of hydrogen peroxide on structure, stability and functional properties of human R12C mutant< alpha>A-Crystallin: the imperative insights into pathomechanism of the associated congenital cataract incidence, Free Radical Biology and Medicine, 89:819-30. IF=5.78

    28) Rezanejad bardeji H, Karbalaei-Heidari H, Rezaei S, Yousefi R (2015) Microsciadin, a New Milk-Clotting Cysteine Protease from an Endemic Species, Euphorbia microsciadia, Biomacromolecular Journal, 1:93-103

    29) Toobaei Z, Yousefi R, Panahi F, Shahidpour S, Nourisefat M, Doroodmand M, Khalafinezhad A (2015) Synthesis of novel poly-hydroxyl functionalized acridine derivatives as inhibitors of alpha-Glucosidase and alpha-Amylase, Carbohydrate Research, 411:22-32. IF=1.81

    30) Yousefi A, Yousefi R, Panahi F, Sarikhani-Khorrami F, Bahaoddini A, Zolghadr A, Khalafinezhad A (2015) Novel curcumin-based pyrano [2, 3-d] pyrimidine anti-oxidant inhibitors for alpha-amylase and alpha-glucosidase; implications for their pleiotropic effects against diabetes complications, International Journal of Biological Macromolecules, 78:46-55. IF=3.13

    31) Shahidpour S, Panahi F, Yousefi R, Nourisefat M, Nabipour M, Khalafinezhad A (2015) Design and synthesis of new antidiabetic alpha-glucosidase and alpha-amylase inhibitors based on pyrimidine-fused heterocycles, Medicinal Chemistry Research, 24:3086-3096. IF=1.43

    32) Ghahramany M, Yousefi R, Khoshaman K, Alavian mehr M (2015) The impact of calcium ion on structure and aggregation propensity of peroxynitrite-modified lens crystallins; new insights into the pathogenesis of cataract disorders, Colloids and Surfaces Biointerface B, 125:170-180. IF=3.90

    33) Yousefi R, Mohammadi R, Taheri-Kafrani A, Sahahsavani M, Dadkhah aseman M, Nabavizadeh S, Rashidi M, Poursasan N, Moosavi Movahedi A (2015) Study of the interaction between two newly synthesized cyclometallated platinum (II) complexes and human serum albumin: Spectroscopic characterization and docking simulation, Journal of Luminescenceis, 159:139-146. IF=2.69

    34) Mohammadi R, Yousefi R, Dadkhah aseman M, Nabavizadeh S, Rashidi M (2015) DNA binding and anticancer activity of novel cyclometalated platinum (II) complexes, Anti-Cancer Agents in Medicinal Chemistry, 15:107-114. IF=2.97

    35) Khalafinezhad A, Panahi F, Yousefi R, Sarrafi S, Gholamalipour Y (2014) Magnetic nanoparticles-supported tungstosilicic acid: as an efficient magnetically separable solid acid for the synthesis of benzoazoles in water, Journal of the Iranian Chemical Society 11:1311-1319.    IF=1.43

    36) Khalafinezhad A, Panahi F, Yousefi R, Gholamalipour Y, Sarrafi S (2014) An efficient procedure for the synthesis of phenacyl and benzyl azolium salts using fluorous alcohols, Journal of the Iranian Chemical Society, 11:1189-1196. IF=1.43

    37) Yousefi R, Alavianmehr M, Mokhtari F, Keshavarz F, Nabavizadeh S, Rashidi M, Moosavi-Movahedi A (2014) Spectroscopic and Molecular Dynamics Studies on Binding of Dimethyplatinum(II) Complex Drug-Based to Human Serum Albumin, Bulletin of the Chemical Society of Japan, 87:1094-1100.  IF=2.21

    38) Alavianmehr M, Yousefi R, Keshavarz F, Mohammad Aghaei D (2014) Probing the Binding of ThioTEPA to Human Serum Albumin, Using Spectroscopic and Molecular Simulation Approaches, Canadian Journal of Chemistry,  92:1066-1073. IF=1.06

    39) Asadi Z, Asadi M, Dehghani firuzabadi F, Yousefi R, Jamshidi M (2014) Synthesis, characterization, anticancer activity,thermal and electrochemical studies of some novel uranyl Schiff base complexes, J of Iranian Chemical Society 11:423-429. IF=1.43

    40) Jamshidi M, Yousefi R, Nabavizadeh S, Rashidi M, Golbon Haghighi M, Niazi A, Moosvi movahedi A (2014) Anticancer activity and DNA-binding properties of novel cationic Pt (II) complexes, International Journal of Biological Macromolecules, 66:86-96. IF=3.13

    41) Pouryasin Z, Yousefi R, Nabavizadeh S, Rashidi M, Hamidizadeh P, Alavian mehr M, Moosvi movahedi A (2014) Anticancer and DNA Binding Activities of Pt(IV) Platinum Complexes; Importance of Leaving Group Departure Rate, Applied Biochemistry and Biotechnology, 172:2604-2617. IF=1.60

    42) Ariaeenejad S, Moosavi-Movahedi A, Kavousi K, Dayer M, Hong J, Yousefi R, Sheibani N, Habibi-Rezaei M (2014) The species and heme pocket properties of Sturgeon hemoglobins upon interaction with n-dodecyl trimethylammonium bromide, Protein and Peptide Letter, 21:171-178. IF=1.068

    43) Aghevlian S, Yousefi R, Faghihi R, Abbaspour A, Niazi A, Jaberipour M, Hosseini A (2013) The improvement of anti-proliferation activity against breast cancer cell line of thioguanine by gold nanoparticles, Medicinal Chemistry Research, 22:303-311.  IF=1.41

    44) Taghavi F, Moosavi-Movahedi A, Bohloolo , Alijanvand H, Salami M, Maghami P, Saboury A, Farhadi M, Yousefi R, Habibi-Rezaei M, Sheibani M (2013) Potassium sorbate as an AGE activator for human serum albumin in the presence and absence of glucose, International Journal of  Biological Macromolecules, 62:146-154. IF=3.13

    45) Keshavarz F, Alavianmehr M, Yousefi R (2013) Molecular interaction of benzalkonium ibuprofenate and its discrete ingredients with human serum albumin, Physical Chemistry Research, 1:111-116.

    46) Panahi F, Yousefi R, Mehraban M, Khalafinezhad A (2013) Synthesis of new pyrimidine-fused derivatives as potent and selective antidiabetic alpha-glucosidase inhibitors, Carbohydrate Research 380:81-91. IF=1.81

    47) Mehraban M, Yousefi R, Taheri-Kafrani A, Panahi F, Khalafinezhad A (2013) Binding study of novel anti-diabetic pyrimidine fused heterocycles to beta-lactoglobulin as a carrier protein, Colloid Surf B Biointerfaces 112:374-379. IF=3.90

    48) Alavi P, Yousefi R, Amirghofran S, Karbalaei-Heidari H, Moosavi-Movahedi A (2013) Structural analysis and aggregation propensity of reduced and non-reduced glycated insulin adducts, Applied Biochemistry and Biotechnology, 170:623-638. IF=1.73

    49) Yousefi R, Khazaei S, Moosavi-Movahedi A (2013) Effect of homocysteinylation on structure, chaperone activity and fibrillation propensity of lens alpha-crystallin, Protein and Peptide Letters, 20:932-941. IF=1.068

    50) Asadi M, Savaripour A, Asadi Z, Ghatee M, Moosavi Baygi F, Yousefi R, Jamshidi M (2013) Synthesis and characterization of some new Schiff base complexes of group 13 elements, ab initio studies, cytotoxicity and reaction with hydrogen peroxide, Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, 101:394-399. IF=2.65

    51) Keshavarz F, Alavian mehr M, Yousefi R (2012) Molecular dynamics simulation and docking studies on the binding properties of several anticancer drugs to human serum albumin, Molecular Biology Research Communications 2:65-73.

    52) Riazi G, Karima O, Khodadadi S, Yousefi R, Mahnam K, Mokhtari F, Cheraghi T, Hoveizi E, Moosavi-Movahedi A (2012) An in vitro study of the role of beta-boswellic acid in the microtubule assembly dynamics, FEBS Letters, 586:4132-4138. IF=3.16

    53) Khazaei S, Yousefi R, Alavian mehr M (2012) Aggregation and fibrillation of eye lens crystallins by homocysteinylation; implication in the eye pathological disorders, Protein Journal 31:717-727. IF=1.029

    54) Yousefi R, Alavian mehr M, Mokhtari F, Panahi F, Mehraban M, Khalafinezhad A (2012) Pyrimidine-fused heterocycle derivatives as a novel class of inhibitors for alpha-glucosidase, Journal of Enzyme Inhibition and Medicinal Chemistry, 380:81-91. IF=3.42

    55) Sharifizadeh A, Saboury A, Moosavi Movahedi A, Maryam S, Yousefi R (2012) A new aspect to chaperone-like activity of bovine beta-casein by protein-protein interactions study, International Journal of Biological Macromolecules, 51:901-907. IF=3.13

    56) Yousefi R, Aghevlian S, Mokhtari F, Samouei H, Rashidi M, Nabavizadeh S, Tavaf langeroudi Z, Pouryasin Z, Niazi A, Faghihi R, Papari M (2012) The anticancer activity and HSA binding properties of thThe anticancer activity and HSA binding properties of the structurally related platinum (II) complexes, Applied Biochemistry and Biotechnology, 167:861-872. IF=1.73

    57) Asadi M, Barzegar S, Asadi Z, Yousefi R, Sa’adi B , Khalili Hezarjaribi H (2012) Synthesis, characterization, and thermodynamic studies of the interaction of some new water-soluble Schiff-base complexes with bovine serum albumin, Journal of Coordination Chemistry, 65:722-739. IF=1.75

    58) Yousefi R, Jalili S, Alavi P, Moosavi Movahedi A (2012) The enhancing effect of homocysteine thiolactone on insulin fibrillation and cytotoxicity of insulin fibril, International Journal of Biological Macromolecules 51:291-298. IF=3.13

    59) Khalili Hezarjaribi H, Yousefi R, Moosavi-Movahedi A (2012) Effect of temperature and ionic strength on structure and chaperone activity of glycated and non-glycated alpha-crystallins, Protein & Peptide Letters 19:450-457. IF=1.068

    60) Yousefi R, Khalafinezhad A, Soltani Rad M, Behrouz , Panahi F, Esmaili M, Ghaffari S, Niazi A, Moosavi-Movahedi A (2011) Structure–cytotoxicity relationship of a novel series of miconazole-like compounds, Medicinal Chemistry Research (MCR) 21:1921-1928. IF=1.41

    61) Salami M, Moosavi-Movahedi A, Moosavi-Movahedi F, Ehsani M, Yousefi R, Farhadi M, Niasari-Naslaji A, Saboury A, Chobert J, Haertle T (2011) Biological activity of camel milk casein following enzymatic digestion, Journal of Dairy Research 78:471-478. IF=1.59

    62) Yousefi R, Jalili S (2011) The synergistic chaperoning operation in a Bi-chaperone system consisting of alpha-crystallin and beta-casein: bovine pancreatic insulin as the target protein, Colloids Surf B Biointerfaces 88:497-504.  IF=3.90

    63) Esmaeili M, Ghaffari S, Moosavi-Movahedi Z, Atri M, Sharifzadeh A, Farhadi M, Yousefi R, Chobert J, Haertle T, Moosavi-Movahedi A (2011) Beta casein-micelle as a nano vehicle for solubility enhancement of curcumin; food industry application, LWT-Food Science and Technology, 44:2166-2172. IF=2.71

    64) Stroylova Y, Zimny J, Yousefi R, Chobert J, Jakubowski H, Muronetz V, Haertle T (2011) Aggregation and structural changes of alpha-S1-, beta- and kappa-caseins induced by homocysteinylation, Biochemica et Biophysica Acta (BBA) 1814:1234-1245. IF=3.016

    65) Miroliaei M, Shirazi M, Yousefi R (2011) A Comparative study on the chaperone-like activity of camel and bovine beta-caseins, Journal of Paramedical Sciences, 2:13-19.

    66) Jalili S, Yousefi R, Moosavi-Movahedi A, Papari M (2011) Effect of Homocysteine Thiolactone on Structure and Aggregation Propensity of Bovine Pancreatic Insulin, Protein Journal, 30:299-307. IF=1.02

    67) Atri M, Saboury A, Yousefi R, Delgalarando M, Chobert J, Haertle T, Moosavi-Movahedi A (2010) Comparative study on heat stability of camel and bovine apo and holo alpha-lactalbumin, Journal of Dairy Research 77:43-49.  IF=1.59

    68) Salami M, Moosavi-Movahedi A, Ehsani M, Yousefi R, Haertle T, Chobert J, Razavi S, Henrich R, Balalaei S, Ebadi S, Poutakdoost S, Niasari-Naslaji A (2010) Improvement of the Antimicrobial and Antioxidant Activities of Camel and Bovine Whey Proteins by Limited Proteolysis, J Agric Food Chem 58:3297-3302. IF=2.85

    69) Karima O, Riazi G, Yousefi R, Moosavi-Movahedi A (2010) The enhancement effect of beta-boswellic acid on hippocampal neurites outgrowth and branching (an in vitro study), Neurological Sciences, 31:315-320. IF=1.73

    70) Gaudin J, Le Parc A, Castrec B, Ropers M, Choiset Y, Shchutskaya J, Yousefi R, Muronetz V, Zuev Y, Chobert J, Heartle T (2009) Engineering of caseins and modulation of their structures and interactions, Biotechnology Advances, 27:1124-1131. IF=9.84

    71) Badraghi J, Moosavi-Movahedi A, Saboury A, Yousefi R, Sharifizadeh A, Hong J, Haertle T, Niasari-Naslaji A, Sheibani N (2009) Dual behavior of sodium dodecyl sulfate as enhancer or suppressor of insulin, International Journal of Biological Macromolecules, 45:511-517. IF=3.13

    72) Salami M, Yousefi R, Ehsani M, Razavi S, Chobert J, Haertle T, Saboury A, Atri M, Niasari-Naslaji A, Ahmad F, Moosavi-Movahedi A (2009) Enzymatic digestion and antioxidant activity of the native and molten globule, International Dairy Journal, 19:518-523. IF=1.93

    73) Badraghi J, Yousefi R, Saboury A, Sharifizadeh A, Haertle T, Ahmad F, Moosavi-Movahedi A (2009) Effect of salts and sodium dodecyl sulfate on chaperone activity of camel [α]S1-CN, Colloids Surf B Biointerfaces 71:300-305. IF=3.90

    74) Yousefi R, Shchutskaya Y, Zimny J, Gaudin J, Moosavi-Movahedi A, Muronetz V, Zuev Y, Chobert J, Haertle T (2009) Chaperone-Like Activities of Different Molecular Forms of beta-casein, Biopolymers, 91:623-632.  IF=2.38

    75) Yousefi R, Gaudin J, Chobert J, Pourpak Z, Moeen M, Moosavi-Movahedi A, Haertle T (2009) Micellisation and immunoreactivities of dimeric beta-caseins, Biophysica Biochemica Acta 1794:1775-1783. IF=3.016

    76) Barzegar A, Yousefi R, Sharifzadeh A, Dalgalarrondo M, Chobert J, Ganjali M, Norozi P, Ehsani M, Niasari-Naslaji A, Saboury A, Haertle T, Moosavi-Movahedi A (2008) Chaperone activities of bovine and camel beta-caseins: Importance of their surface hydrophobicity in protection against alcohol dehydrogenase aggregation, International Journal of Biological Macromolecules, 42:392-399. IF=3.13

    77) Zali H, Rezaei-Tavirani M, Kariminia A, Yousefi R, Shokrgozar M (2008) Evaluation of growth inhibitory and apoptosis inducing activity of human calprotectin on the human gastric cell line, Iran Biomed J, 12:7-14.

    78) Hassanisadi M, Barzegar A, Yousefi R, Delgalarando M, Chobert J, Haertle T, Saboury A, Moosavi-Movahedi A (2008) Chemometric study of the aggregation of alcohol dehydrogenase and its suppression by beta-caseins: a mechanistic perspective, Analytica Chimica Acta, 613:40-47. IF=4.70

    79) Amani M, Yousefi R, Moosavi-Movahedi A, Pintus , Mura A, Floris , Kurganov B, Saboury A (2008) Structural changes and aggregation process of cu/containing amine oxidase in the presence of 2,2,2'-trifluoroethanol, Protein Pept Lett, 15:521-527. IF=1.068

    80) Salami M, Yousefi R, Ehsani M, Dalgalarrondo M, Chobert J, Haertle T, Razavi S, Saboury A, Niasari-Naslaji A, Moosavi-Movahedi A (2008) Kinetic characterization of hydrolysis of camel and bovine, International Dairy Journal, 18:1097-1102. IF=1.93

    81) Yousefi R, Imani M, Kabudanian-Ardestani S, Saboury A, Gheibi N, Ranjbar B (2007) Human Calprotectin: Effect of Calcium and Zinc on its Secondary and Tertiary, Acta Biochimica et Biophysica Sinica, 39:795-802. IF=2.21

    82) Divsalar A, Saboury A, Yousefi R, Moosavi-Movahedi A, Mansori-Torshizi H (2007) Spectroscopic and Cytotoxic Study of the Novel Designed Palladium Complexes: alpha-lactoglobulin and K562 as the Targets, Int J Biol Macromolecules, 40:381-386. IF=3.13

    83) Yousefi R, Kabudanian-Ardestani S, Imani M, Saboury A, Kariminia A (2007) ROS Induction by Human Calprotectin in K562 and the Reversal Effect of Vitamin E, Research Journal of Biological Sciences, 2:215-220.

    84) Moosavi-Movahedi A, Amani M, Moosavi Nejad S, Hashemnia S, Ahmad F, Floris , Mura A, Rezaei-Tavirani M, Hakimelahi G, Saboury A, Yousefi R (2007) Thermal dissection of lentil seedling amine oxidase domains by differential scanning calorimetry, Biosci Biotechnol Biochem, 71:1644-1649. IF=1.17

    85) Moosavi-movahedi A, Pirzadeh , Hashemnia S, Ahmadian S, Hemmateenejad B, Amani M, Saboury A, Ahmad F, Shamsipour M, Hakimelehi G, Tsai F, Yousefi R, Alijanvand H (2007) Fibril formation of lysozyme upon interaction with sodium dodecyl sulfate at pH 9.2, Colloids Surf B Biointerfaces 60:55-61. IF=3.90

    86) Yousefi R, Kabudanian-Ardestani S, Saboury A, Kariminia A, Zeinali M, Amani M (2005) Investigation on the Surface Hydrophobicity and Aggregation Kinetics of Human Calprotectin in the Presence of Calcium, J Biochem Mol Biol, 38:407-413.  IF=2.0

    87) Yousefi R, Saboury A, Ghadermarzi M, Moosavi-Movahedi A (2000) Effects of cysteine on the inactivation of bovine liver catalase, Bull Korean Chem Soc, 21:567-570.  IF=0.79

     

     

     

     

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Presentation

Yousefi R "The pleiotropic preventive effects of curcumin in diabetes mellitus; the insights into attenuation of postprandial hyperglycemia and downstream molecular events " Annual International Conference on Biology (2015)

Shahsavani M, Nabavizadeh S, Rashidi M, Yousefi R "In silico study of the interaction between four newly synthesized platinum complexes and human serum albumin " The national conference on protein and peptide sceinces (2014)

Khazaei S, Javadi S, Sasan Moghadam S, Yousefi R "Decreasing chaperone activity and change in the native structure of alpha-crystallin upon homocysteinylation " The national conference on protein and peptide sciences (2014)

Ghahramany M, Yousefi R "Calcium indicates significant destabilizing effect on peroxynitrite-modified lens crystallins " The national conference on protein and peptide sciences (2014)

Khoshaman K, Ghahramany M, Javadi S, Yousefi R "The role of disulfide bridge covalent cross-linking on thestructure, chaperone activity and aggregation propensity of wild-type and R12C mutant alpha A-crystallins " The national conference on protein and peptide sciences (2014)

Mehraban M, Moafiyan Z, Taheri-Karfrani A, Panahi F, Khalafinezhad A, Yousefi R "-Beta-lactoglobulin; a new transport vehicle for anti-diabetic drugs " The national conference on protein and peptide sceinces (2014)

Toobaie Z, Yousefi R, Panahi F, Khalafinezhad A "Molecular docking study of the interaction between poly hydroxyl functionalized acridine derivatives and beta-Lactoglobulin " The national conference on protein and peptide sceinces (2014)

Shahidpour S, Yousefi R, Khalafinezhad A, Panahi F "Antidiabetic pyrimidine-fused heterocyclic compounds as low risk inhibitors for alpha-amylase and alpha-glucosidase " The national conference on protein and peptide sceinces (2014)

Yousefi A, Yousefi R, Panahi F, Khalafinezhad A "Binding properties of curcumin-based pyrano [2, 3.D] pyrimidine derivatives to beta-lactoglobulin as a carrier protein " The national conference on protein and peptide sceinces (2014)

Zafaranchi zadeh moghadam S, Ghahramany M, Sasan moghadam S, Yousefi R "Study on the role of calcium ion on structure and aggregationproperties of eye lens crystallins " The national conference on protein and peptide sceinces (2014)

Panahi F, Khalafinezhad A, Yousefi R "Pyrimidine-fused heterocycle derivatives as potent and selective antidiabetic alpha-glucosidase inhibitors " The national conference on protein and peptide sceinces (2014)

Mehraban M, Moafiyan Z, Panahi F, Khalafinezhad A, Yousefi R "Recent discovery of alpha-glucosidase inhibitors with pyrimidine scaffold " The national conference on protein and peptide sceinces (2014)

Yousefi R, Khazaei S, Ghahramany M, Tavaf langeroudi Z "The impact of homocyteinylation and peroxynitrytation on structure, function and aggregation of lens: implications for their possible contribution in the pathogenesis of cataract disorders ; " The national conference on protein and peptide sceinces (2014)

Alavi P, Yousefi R "Non-enzymatic glycation of insulin reduces its propensity for aggregation " 1 st Tabriz International Life Sceince Conference , 12 th Iran Biophysical Conference (2013)

Khazaei S, Yousefi A, Yousefi R "Homocysteinylation of lens Crystallins: From Protein Aggregation to Possible Risk Factor in Development of Cataract Disorders. " 1st Tabriz International Life Sceince Conference , 12 th Iran Biophysical Conference (2013)

Zafaranchi zadeh moghadam S, Yousefi R "Non-Enzymatic Glycation Protects Calcium Induced total lens soluble Proteins Aggregation " 1st Tabriz International Life Sceince Conference , 12 th Iran Biophysical Conference (2013)

Zafaranchi zadeh moghadam S, Yousefi R, Saba S, Ragerdi kashani M, Barzegar A "Prediction of Gamma-B Crystallin Stability Upon Point Mutation at Phe56 " The First International & 11th Iran Biophysical Chemistry Conference (2012)

Yousefi R, Jalili S, Alavi P "Applying a Bi-chaperone System to Prevent Insulin Aggregation " The First International & 11th Iran Biophysical Chemistry Conference (2012)

Yousefi R, Ferdosi L, Zamani H, Kavosipour S, Shahsavani M "Role of Low Molecular Weight Biomolecules in Antioxidant Profile of Camel and Bovine Milk " The First International & 11th Iran Biophysical Chemistry Conference (2012)

Yousefi R, Alavi P, Jalili S, Khabbaz-Shirazi M, Moosavi-Movahedi A "Homocysteine Thiolactone Induces Insulin Fibrillation and Enhances Cytotoxic Properties of Insulin Fibrils " The First International & 11th Iran Biophysical Chemistry Conference (2012)

Toobaei Z, Mehraban M, Yousefi R, Panahi F, Khalafinezhad A, Mokhtari F, پاپري "Aromatic Substitution on the Pyrimidine-Fused Heterocycle Molecules Significantly Enhances Their Inhibitory Properties Against Alpha-Glucosidase " The First International & 11th Iran Biophysical Chemistry Conference (2012)

Taheri B, Jalili S, Yousefi R, Alavi P, Moosavi Movahedi A "Homocysteinylation of Bovine Pancreatic Insulin, Importance of C-Terminal Beta Chain In Structural Stability and Protein Aggregation " The First International & 11th Iran Biophysical Chemistry Conference (2012)

Saba S, Yousefi R, Ragerdi kashani M, Zaafaranchi zade moghadam S "The Paradigm of Uncertain Role of Surface Hydrophobicity in Chaperone Activity of Alpha-Crytallins " The First International & 11th Iran Biophysical Chemistry Conference (2012)

Mohammadi R, Yousefi R, Pouryasin Z, Aghevlian S, Samouei H, Rashidi M, Nabavizadeh S, Faghihi R "The Anticancer Activity and DNA/HSA Binding Properties of Benzo [H] Quinoline (Bhq) Pt (II) Complexes " The First International & 11th Iran Biophysical Chemistry Conference (2012)

Mehraban M, Yousefi R, Panahi F, Khalafinezhad A "Mammalian Intestinal Alpha Glucosidase Inihibitory Activity of Novel Pyrimidine Fused Heterocycles " The First International & 11th Iran Biophysical Chemistry Conference (2012)

Jamshidi M, Yousefi R, Nabavizadeh S, Golbon Haghighi M, Moosavi Movahedi A "Study on the Effect of Newly Synthesized Cateionic Pt (II)-complexes on Structure and Binding Properties of Human Serum Albumin " The First International & 11th Iran Biophysical Chemistry Conference (2012)

Hajari S, Yousefi R, K. ardestani S "The Enhancement of Surface Hydrophobicity by Calcium Ion may have Significant Contribution in cell Death Inducing " The First International & 11th Iran Biophysical Chemistry Conference (2012)

Aghevlian S, Tavaf langeroudi Z, Mohammadi R, Faghihi R, Yousefi R "Gold Nanoparticle as a Drug Delivery System for Thiol-Containing Purine Antimetabolites " The First International & 11th Iran Biophysical Chemistry Conference (2012)

Shahsavani M, Yousefi R "The restrict avoidance is the only medication for cow’s milk protein allergy so far " The First Seminar On The Role Of Basic Sciences On The Health Promotion (2011)

Mokhtari F, Papari M, Yousefi R "Alpha-Glucosidase Inhibitors for Patients with Diabetes Mellitus; Challenges and Hopes " The First Seminar On The Role Of Basic Sciences On The Health Promotion (2011)

Pouryasin Z, Kabudanian-Ardestani S, Yousefi R "Is Calprotectin a Proper Biomarker for Gut Inflammation " The First Seminar On The Role Of Basic Sciences On The Health Promotion (2011)

Khalafinezhad A, Panahi F, Yousefi R "The Chemistry behind Reduction of Diabetes Mellitus Complications; Role of Polyhydroxy Functionalized Pyrimidine-fused Heterocycles " 15th Iranian Chemistry Congress (ICC 2011) (2011)

Yousefi R "The inhibitors and breakers of protein-protein AGE cross-links; " meli (2011)

Khalafinezhad A, Panahi F, Khalili hezar jaribi H, Yousefi R "The inhibitors and breakers of protein-protein AGE cross-links " Journal of the Iranian Chemical Society (2010)

Khalili hezar jaribi H, Yousefi R "Salt and temperature dependent chaperone activity of alpha-crystallin " 10th Iran Biophysical Chemistry Conference, 22-23 February 2011. (2010)

Salami M, Moosavi movahedi F, Ehsani M, Yousefi R, Niasari-Naslaji A, Moosavi movahedi A "ACE inhibitory effect of bioactive peptides from camel milk " 10th Iran Biophysical Chemistry Conference, 22-23 February 2011 (2010)

Yousefi R, Jalili S, Papari M, Moosavi-Movahedi A "Homocysteine thiolactone induces gross structural alteration " 10th Iran Biophysical Chemistry Conference (2010)

Jalili S, Yousefi R "Alpha-crystallin and beta-casein prevent insulin aggregation " 10th Iran Biophysical Chemistry Conference, 22-23 February 2011. (2010)

Alavi P, Tavaf langeroudi Z, Shahsavani M, Yousefi R "Protein biomarkers of amniotic fluid for identification of pregnancy associated abnormalities " The National Proteomics Congress, Shiraz University, 28-29 Oct 2010 (2010)

Shahsavani M, Hakimara M, Raeesi Z, Yousefi R "The clinical application of biomarkers in proteomics " The National Proteomics Congress, Shiraz University, 28-29 Oct 2010 (2010)

Jalili S, Yousefi R "The pathological consequences of protein homocysteinylation " The National Proteomics Congress, Shiraz University, 28-29 Oct 2010 (2010)

Pouryasin Z, Yousefi R "Calprotectin, a novel biomarker with remarkable sensitivity and specificity for inflammatory disorders " The National Proteomics Congress, Shiraz University, 28-29 Oct 2010 (2010)

Kavosipour S, Zamani H, Shahsavani M, Niazi A, Yousefi R "Study on the antioxidant activity of bovine and donkey milk and milk protein fractions " The National Proteomics Congress, Shiraz University, 28-29 Oct 2010 (2010)

Yousefi R, Moosavi movahedi A, Haertle T "IgE-mediated immune reactivity of milk proteins; role of phosphorylation and homocysteinylation " The National Proteomics Congress, Shiraz University, 28-29 Oct 2010 (2010)

Mokhtari F, Papari M, Yousefi R "Inhibition of alpha-glucosidases as one of the most practical approach to reduce hyperglycemia associated toxicities in diabetes mellitus " The National Proteomics Congress, Shiraz University, 28-29 Oct 2010 (2010)

Khalafinezhad A, Panahi F, Yousefi R "Synthetic inhibitors of protein glycation a novel approach to reduce protein toxicity " The National Proteomics Congress, Shiraz University, 28-29 Oct 2010 (2010)

Yousefi R, Khalili hezar jaribi H, Barzegar A, Zamani H, Shahsavani M, Haertle T, Moosavi-Movahedi A "Chaperone-Like Activity of Beta-Casein; a Mechanistic " 9th Iran Biophysical Chemistry Conference (2010)

Behrooz S, Yousefi R, Khalafinezhad A, Sultani M, Esmaeili M, Moosavi-Movahedi A "Synthesis of novel series of cytotoxic imidazole-based " 9th Iran Biophysical Chemistry Conference (2010)

Yousefi R, Zamani H, Tavaf-Lagroudi Z, Valifard M, Khalili hezar jaribi H, Haertle T, Moosavi-Movahedi A "A Novel Approach to Quantify the Chaperone-Like activity " 9th Iran Biophysical Chemistry Conference (2010)

Yousefi R, Khalili hezar jaribi H, Tavaf-Langroudi Z, Zamani H, Haertle T, Moosavi-Movahedi A "Impact of the Major Phosphorylation Site on Chaperoning " 9th Iran Biophysical Chemistry Conference (2010)

Salami M, Ehsani M, Khaki-Najafabadi I, Moosavi-Movahedi F, Yousefi R, Saboury A, Moosavi-Movahedi A "Antioxidant peptides derived from camel casein " 9th Iran Biophysical Chemistry Conference (2010)

Esmaeili M, Ghaffari S, Moosavi-Movahedi Z, Sharifizadeh A, Yousefi R, Farhadi M, Moosavi-Movahedi A "Binding of curcumin to beta casein, a route to make a functional food " 9th Iran Biophysical Chemistry Conference (2010)

Yousefi R, Haertle T, Chobert J, Moosavi-Movahedi A "Effect of position polarity of cysteine substitution on the " 10th Iranian Congress of Biochemistry & 3rd International Congress of Biochemistry and Molecular Biology (2009)

Yousefi R, Barzegar A, Haertle T, Chobert J, Moosavi-Movahedi A, Saboury A, Sharifizadeh A, Hasani Saadi M "Analysis of reversible interaction between beta-casein " 10th Iranian Congress of Biochemistry & 3rd International Congress of Biochemistry and Molecular Biology, (2009)

Balverdi M, Salami M, Yousefi R, Safari M, Habibi-Rezaei M, Moosavi-Movahedi A "Antioxidant activity of bovine whey-based kombucha " 0th Iranian Congress of Biochemistry & 3rd International Congress of Biochemistry and Molecular Biology (2009)

Atri M, Saboury A, Yousefi R, Moosavi-Movahedi A "A comparative study on the stability of bovine and camel " 10th Iranian Congress of Biochemistry & 3rd International Congress of Biochemistry and Molecular Biology (2009)

Salami M, Yousefi R, Ehsani M, Saboury A, Moosavi-Movahedi A "Effect of calcium binding on the functional properties of camel " 10th Iranian Congress of Biochemistry & 3rd International Congress of Biochemistry and Molecular Biology (2009)

Shirazi M, Miroliaei M, Yousefi R, Moosavi-Movahedi A, Aeidi A "Beta-caseins inhibit the formation of amyloid fibrils in apo-forms " 10th Iranian Congress of Biochemistry & 3rd International Congress of Biochemistry and Molecular Biology (2009)

Editorial Board Member of Scientific Journals

1) Iran Society of Biophysical Chemistry (ISOBC).

2) Iran Peptide Scocity (IPS).

3) Academic Member of Athens Institute for Education and Research.

 

Professional Membership

1) PLOS ONE (Academic editor)

2) International Journal of Biochemistry and Biophysics

3) Enzyme Engineering

4) Journal of Metabolic Investigation

5) Iranian Journal of Science and. TechnologyTransaction AScience