Dr. Reza Yousefi (Associate Professor of Biochemistry)

Department
Biology
About

Professional Summary

- Our research group is interested in the role of mutation and chemical modification on structural/functional variation and aggregation of lens crystallins and human insulin. Crystallin proteins are pathogenic targets for various chemical modifications and mutations which ultimately affect the proper function and transparency of eye lens (Fig.1). Stability and aggregation of insulin is currently a big medical and biotechnological challenge (Fig.2). Site-directed mutagenesis is an important technique to obtain new insulin molecules with increased stability and lower propensity for aggregation.

- We are interested also in the inhibitory potential of synthetic ligands against alpha-glucosidase and alpha-amylase enzymes which are potential targets for controlling post-prandial hyperglycemia in diabetic patients (Fig.3).

- Other research direction in our lab is to examine anticancer and DNA/protein binding properties of novel synthetic platinum complexes. The anticancer activity of Pt complexes is believed to be result of their interaction to cellular DNA and their ability to distort  DNA structure. Also, non-specific interaction of these complexes with protein can explain their potential side effects  (Fig.4)

- We are using various techniques including cloning, mutagenesis and different types of chromatography, UV-Vis spectroscopy, fluorescence measurement, circular dichroism (CD), differential scanning calorimetery (DSC), dynamic light scattering (DLS), various types of electrophoretic assessments and western blot, microscopic visualization and ELISA, insilco analysis and cell culture techniques in our studies. All of these experiments require contributions from the disciplines of  biochemistry, molecular biology, chemical biology and organic chemistry.

Research group

 

PhD students 

Kazem Khoshaman

General topic: Role of mutation in structure and function   of recombinant alphaA-crystallin.

 

Maryam Ghahramani 

General topic: Role of mutation in structure and function of recombinant alpha B-crystallin.

 

 

Mohsen Akbarian

General topic: Role of mutation in structure, stability and aggregation of recombinant insulin.

 

Musa Amirahmadi

General topic: Concurrent trainig and lens opacity: The preventive role of antioxidant compounds

 

                  Mohammad Bagher Shahsavani

 General topic: Role of mutation in structure, stability and  aggregation of recombinant insulin.

MSc students

 

Mahtab Hafizi

General topic: Role of mutation in structure and function of recombinant alpha B-crystallin.

Pezhman Ashou

General topic: Anticancer activity of Platinum complexes and their interaction with HSA and DNA.

 

 

 

 

 

 

 

 

Collaborations

A) Local collaboration

1) Professor A. Khalafi-Nezhad (Department of Chemistry, Shiraz University).

2) Professor M. Rashidi (Department of Chemistry, Shiraz University).

3) Professor S.M. Nabavizadeh (Department of Chemistry, Shiraz University).

4) Professor A.Oryan (Department of Pathology, School of Veterinary Medicine, Shiraz University).

5) Dr. A.Niazi (Institute of Biotechnology,  Shiraz University)

6) Professor M.M. Alavianmehr (Department of Chemistry, Shiraz University of Technology).

7) Professor Y.Ghasemi (Department of Pharmaceutical Biotechnology, Shiraz University of Medical Sceinces).

8) Dr. A.M Tamaddon (Department of Pharmaceutical Biotechnology, Shiraz University of Medical Sceinces).